Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin.

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Ultrasound-accelerated immunoassay, as exemplified by enzyme immunoassay of choriogonadotropin.

The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with a...

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DETERMINATION OF SERUM CORTISOL LEVEL BY A DIREC T ENZYME IMMUNOASSAY USING PENICILLINASE AS LABEL

Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...

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determination of serum cortisol level by a direc t enzyme immunoassay using penicillinase as label

serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...

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Detection of poliovirus antigen by enzyme immunoassay.

A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984. The EIA was highly specific for poliovirus type 3, and it was about 30 times ...

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Enzyme immunoassay for oxytocin.

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 1984

ISSN: 0009-9147,1530-8561

DOI: 10.1093/clinchem/30.9.1446